Best primers for kinship detection of the 8 main parents of indica hybrid rice in the south China by InDel.

PrimerID nAmp AmpPos Fprimer Rprimer FpTm RpTm AmpSize TF-B FT-B WF-B GH1521 GH998 GH138 HHZ YHSM


Note:
PrimerID: primer ID; nAmp: number of potential amplicons; AmpPos: amplicon position on reference genome; Fprimer: forward primer; Rprimer: reverse primer; FpTm: Tm of forward primer; RpTm: Tm of reverse primer; AmpSize: amplicon size (bp); TF-B: TaifengB; FT-B: FengtianB; WF-B: WufengB; GH1521:Guanghui1521; GH998: Guanghui998; GH138: Guanghui138; HHZ: Huanghuazhan; YHSM: Yuehesimiao; The reference genome is MSU-7.0 (http://rice.uga.edu/). The genotype of 8 main parent, TF-B, FT-B, WF-B, GH1521, GH998, GH138, HHZ, and YHSM are given as “012-1”, which 0 is reference homozygote genotype, 1 is heterozygosis genotype, 2 is mutant homozygote genotype, and -1 present failed detection in the allele in that variety. The InDel variations were identified by next genomic sequencing with average coverage larger than 40×.

本站为个人学习练习记录,资料均来自公开文献,仅供学习查阅使用,属非经营性个人网站,不做任何商业用途。
最后更新:2026 年 11 月
备案编号:粤ICP备2026172674号