Best primers for kinship detection of the 8 main parents of indica hybrid rice in the south China by InDel.
| PrimerID | nAmp | AmpPos | Fprimer | Rprimer | FpTm | RpTm | AmpSize | TF-B | FT-B | WF-B | GH1521 | GH998 | GH138 | HHZ | YHSM |
|---|
Note:
PrimerID: primer ID; nAmp: number of potential amplicons; AmpPos: amplicon position on reference genome; Fprimer: forward primer; Rprimer: reverse primer; FpTm: Tm of forward primer; RpTm: Tm of reverse primer; AmpSize: amplicon size (bp); TF-B: TaifengB; FT-B: FengtianB; WF-B: WufengB; GH1521:Guanghui1521; GH998: Guanghui998; GH138: Guanghui138; HHZ: Huanghuazhan; YHSM: Yuehesimiao; The reference genome is MSU-7.0 (http://rice.uga.edu/). The genotype of 8 main parent, TF-B, FT-B, WF-B, GH1521, GH998, GH138, HHZ, and YHSM are given as “012-1”, which 0 is reference homozygote genotype, 1 is heterozygosis genotype, 2 is mutant homozygote genotype, and -1 present failed detection in the allele in that variety. The InDel variations were identified by next genomic sequencing with average coverage larger than 40×.
